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COLUMBIA AGAR BASE EU-PH-USP

For the isolation and cultivation of fastidious microorganisms

COLUMBIA AGAR BASE EUROPEAN PHARMACOPOEIA, USP (ISO 10272) - 500 g

For the isolation and cultivation of fastidious microorganisms and the determination of hemolytic reactions

Applications

  • Media for general use
  • Hemolytic Activity
  • OTHER APPLICATIONS

Packaging

Suspend 42.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121°C for 15 minutes. The medium is generally enriched with sterile defibrinated blood (sheep, rabbit or horse), serum or some other material. Cool to 45-50ºC and aseptically add 5-10% sterile defibrinated blood, homogenize gently and pour into Petri dishes. Be careful to avoid bubble formation when adding the blood.

 Formula

  • Pancreatic Digest of Casein 10
  • Yeast Extract 5
  • Meat Peptic Digest 5
  • Sodium Chloride 5
  • Heart Pancreatic Digest 3
  • Maize Starch 1
  • Bacteriological Agar 13.5

Recommended supplements (to be purchased separately)

  • CNA (STAPH/STREP) SUPPLEMENT - Selective supplement for Staphylococci, Streptococci and Pneumococci isolation
  • BRUCELLA SUPPLEMENT - Selective supplement for Brucella isolation

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