Choose your language: it-IT en-US
Facebook Twitter Instagram
« Come back | You are in: Skip Navigation Links Home » Life Science » Microbiology » Dehydrated culture media CD Series » Dehydrated culture media - E »   EOSIN METHYLENE BLUE AGAR E M B 500 g

EOSIN METHYLENE BLUE AGAR (E.M.B.) - 500 g

For the isolation, cultivation and differentiation of gram negative enteric bacilli

EOSIN METHYLENE BLUE AGAR (EMB) - 500 g

For the isolation, cultivation and differentiation of gram negative enteric bacilli from clinical and other specimens 

Applications

  • Isolation / Cultivation / Differentiation
    COLIFORMS
    ENTEROBACTERIA

Packaging

Suspend 36 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC, mix well, avoiding the formation of bubbles and dispense carefully into Petri Dishes. DO NOT OVEARHEAT. The prepared medium should be stored at 8-15°C. The color is tournasol blue. Sterilization reduces the methylene blue, leaving the medium orange in color. The normal purple may be restored by gently mixing. The reduced medium should be shaken to oxidize the methylene blue; otherwise a dark zone from the top extending downwards will gradually appear.

Formula

  • Bacteriological Peptone 10
  • Lactose 5
  • Sucrose 5
  • Dipotassium Phosphate 2
  • Eosin Y 0.4
  • Methylene Blue 0.065
  • Bacteriological Agar 13.5

You might also be interested in these items
all rights reserved © 2017 CHEIMIKA SAS DI HEIM JURGENYour purchases are guaranteed by the major lenders
Powered by NapoliWeb S.r.l. - Web Agency Napoli Web Agency Napoli