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EasyScript One-Step gDNA Removal and cDNA Synthesis SuperMix

Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination

EasyScript One-Step gDNA Removal and cDNA Synthesis SuperMix

Description

Unique genomic DNA remover is combined with EasyScript First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis.

After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.

Highlights

  • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination
  • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
  • cDNA up to 8 kb

Applications

Multiple copy gene detection

Storage

At -20°C for two year

Kit Contents

Component

TS/AE311/02 (50 rxns)

TS/AE311/03 (100 rxns)

EasyScript RT/RI Enzyme Mix

50 μl

100 μl

gDNA Remover

50 μl

100 μl

2×ES Reaction Mix

500 μl

1 ml

Random Primer(N9) (0.1 μg/μl)

50 μl

100 μl

Anchored Oligo(dT)18 Primer (0.5 μg/μl)

50 μl

100 μl

RNase-free Water

500 μl

1 ml


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