MANNITOL SALT AGAR (MSA)

For the isolation and enumeration of pathogenic staphylococci

MANNITOL SALT AGAR (MSA) (CHAPMAN MEDIUM) EUROPEAN PHARMACOPOEIA, USP - 500 g

For the isolation and enumeration of pathogenic staphylococci from clinical samples and other materials

Applications

Isolation / Enumeration
STAPHYLOCOCCUS

Packaging

Suspend 111 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC, mix well and dispense into plates.

Formula

Sodium Chloride 75
D-Mannitol 10
Pancreatic Digest of Casein 5
Peptic Digest of Animail Tissue 5
Beef Extract 1
Phenol Red 0.025
Bacteriological Agar 15

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