2×TransTaq High Fidelity（HiFi）PCR SuperMix I (-dye )
Ready-to-use mixture of TransTaq High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer
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2×TransTaq High Fidelity（HiFi）PCR SuperMix I (-dye )
Description
TransTaq High Fidelity (HiFi) PCR SuperMix I is a ready-to-use mixture of TransTaq High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer.
TransTaq High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA.
The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.
• TransTaq High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq DNA Polymerase
• Extension rate is about 1-2 kb/min
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors
• Amplification of genomic DNA fragment up to 15 kb
Applications
• Complex templates
• GC/AT-rich templates
• Long PCR
• High yield PCR
Storage
At -20℃ for two years
Kit Contents
|
Component
|
TS/AS131/01
|
TS/AS131/02
|
|
2×TransTaq HiFi PCR SuperMix I
|
1 ml
|
5×1 ml
|
|
ddH2O
|
1 ml
|
5 ml
|
Reaction System
|
Component
|
Volume
|
Final Concentration
|
|
Template
|
Variable
|
As required
|
|
Forward Primer (10 μM)
|
1 μl
|
0.2 μM
|
|
Reverse Primer (10 μM)
|
1 μl
|
0.2 μM
|
|
2×TransTaq HiFi PCR SuperMix I
|
25 μl
|
1×
|
|
ddH2O
|
Variable
|
-
|
|
Total volume
|
50 μl
|
-
|