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2×EasyTaq PCR SuperMix (+dye)

Ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs and optimized buffer.

2×EasyTaq PCR SuperMix  ( +dye )

Description

EasyTaq PCR SuperMix is a ready-to-use mixture of EasyTaq DNA Polymerase, dNTPs and optimized buffer.

The SuperMix is provided at 2×concentration and used at 1× concentration by adding template, primers and H2O.

PCR products are not suitable for PAGE.

  • Extension rate is about 1-2 kb/min
  • Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY-T vectors
  • Amplification of genomic DNA fragment up to 4 kb

Application

Routine PCR

Storage

at -20°C for two years

Kit Contents

Code

TS/AS111/11

TS/AS111/12

TS/AS111/13

TS/AS111/14

2×EasyTaq PCR SuperMix (+dye) 

1 ml

5×1 ml

15×1 ml

6×80 ml 

ddH2O

1 ml

5 ml

3×5 ml

96×5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×EasyTaq PCR SuperMix

25 μl

ddH2O

Variable

-

Total volume

50 μl

-


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