2×TransTaq High Fidelity（HiFi）PCR SuperMix II (-dye )
Ready-to-use mixture of TransTaq High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer
2×TransTaq High Fidelity（HiFi）PCR SuperMix II (-dye)
Description
TransTaq High Fidelity (HiFi) PCR SuperMix II is a ready-to-use mixture of TransTaq High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer.
TransTaq High Fidelity (HiFi) PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.
• TransTaq High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase
• Extension rate is about 1-2 kb/min
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY-T vectors
• Amplification of genomic DNA fragment up to 15 kb
Applications
• Complex templates
• GC/AT-rich templates
• Long PCR
• High yield PCR
Storage
At -20℃ for two years
Kit Contents
|
Component
|
TS/AS131/21
|
TS/AS131/22
|
|
2×TransTaq HiFi PCR SuperMix II
|
1 ml
|
5×1 ml
|
|
ddH2O
|
1 ml
|
5 ml
|
Reaction System
|
Component
|
Volume
|
Final Concentration
|
|
Template
|
Variable
|
As required
|
|
Forward Primer (10 μM)
|
1 μl
|
0.2 μM
|
|
Reverse Primer (10 μM)
|
1 μl
|
0.2 μM
|
|
2×TransTaq HiFi PCR SuperMix II
|
25 μl
|
1×
|
|
ddH2O
|
Variable
|
-
|
|
Total volume
|
50 μl
|
-
|